Reduced pinosome formation in a cell-fusion-impaired mutant of mouse lymphocytic cell line L5178Y
✍ Scribed by Dr. Peter G. Pauw; Karen G. Biagi; Joan K. Stadler
- Publisher
- John Wiley and Sons
- Year
- 1986
- Tongue
- English
- Weight
- 653 KB
- Volume
- 126
- Category
- Article
- ISSN
- 0021-9541
No coin nor oath required. For personal study only.
✦ Synopsis
Accumulation and release of a fluid-phase marker ([14C]-sucrose) were studied in a subline of the mouse lymphocytic cell line L5178Y and in a polyethylene glycol-resistant, intercellular-fusion-impaired mutant of this line. The mutant was found to accumulate [14C]-sucrose at a significantly slower rate than t h e parent. Analysis of release of preloaded label shows that the reduced rate of accumulation is due to a correspondingly low level of internalization, i.e., pinosome formation, rather than to a reduction in delivery of label to lysosomes. Cell-to-cell fusion and pinosome formation both involve a fusion event initiated at the extracellular surface of the plasma membrane, and we propose that the coordinate reduction in both processes suggests that they are mechanistically related.