The substrate specificity factor, VcKo/ V o Kc, of spinach (Spinacia oleracea L.) ribulose 1,5-bisphosphate carboxylase/oxygenase was determined at ribulosebisphosphate concentrations between 0.63 and 200 gM, at pH values between 7.4 and 8.9, and at temperatures in the range of 5 ~ C to 40 ~ C. The
Reduced CO2/O2Specificity of Ribulose-Bisphosphate Carboxylase/Oxygenase in a Temperature-Sensitive Chloroplast Mutant of Chlamydomonas
โ Scribed by Zhixiang Chen, Chris J. Chastain, Souhail R. Al-Abed, Raymond Chollet and Robert J. Spreitzer
- Book ID
- 123635684
- Publisher
- National Academy of Sciences
- Year
- 1988
- Tongue
- English
- Weight
- 806 KB
- Volume
- 85
- Category
- Article
- ISSN
- 0027-8424
- DOI
- 10.2307/31854
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An improved anion-exchange chromatographic method for determining the carboxylation/oxygenation specificity (tau) of ribulose 1,5-bisphosphate carboxylase/oxygenase is presented. This assay, which entails radiometric detection of [1-3H]ribulose-bisphosphate turnover products separated on MonoQ anion
The Chlamydomonas reinhardtii (Dangeard) temperature-conditional mutant 68-11AR is phenotypically indistinguishable from the wild type at the permissive temperature (25~ but has greatly reduced photosynthetic ability and requires acetate for growth at the restrictive temperature (35~ The mutant str