Reduced-bore monolithic silica column modified with C8-TEOS for reversed-phase electrochromatography

✍ Scribed by Qishu Qu; Rongjun Qu; Qiang Xu; Yubao Wang; Xiaorong Wang; Youzhao He

• Publisher: John Wiley and Sons
• Year: 2004
• Tongue: English
• Weight: 156 KB
• Volume: 27
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.200301699

✦ Synopsis

Abstract

Monolithic silica columns of 2.7 mm ID were prepared and derivatized with C~8~‐TEOS and TEOS by on‐column sol‐gel reaction. These C~8~ large diameter monolithic silica columns gave 21 000 theoretical plates for aromatic hydrocarbons in 60% acetonitrile and 40% Tris‐HCl buffer. The surface areas as well as the separation reproducibility were improved on coating by the sol‐gel approach. Joule heating was greatly reduced by using monolithic columns to which fine quartz sand had been added during column preparation. Since this is a preliminary investigation on a monolithic column with such a large inner diameter, the separation efficiency was not so high as that presently achieved in normal capillary electrochromatography (CEC). However, use of the columns improved sample loadability and concentration detectability of electrochromatography, and semi‐preparative separations could be performed.