Redox properties of 2[4Fe4S] ferredoxins

✍ Scribed by Eugene T. Smith; Dennis W. Bennett; Benjamin A. Feinberg

• Book ID: 102982381
• Publisher: Elsevier Science
• Year: 1991
• Tongue: English
• Weight: 596 KB
• Volume: 251
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(91)87111-j

✦ Synopsis

The redox properties of the 2[4Fe-4S] ferredoxin isolated from Clostridium pasteurianum were examined directly at an edge pyrolytic graphite electrode using square-wave voltammetry.

The direct electrochemical approach was compared with other techniques that have been used to determine the equilibrium reduction potential of this protein. A microcell is described that permits routine electrochemical experiments on small sample volumes (25-50 ~1). The electrostatic potentials of a homologous ferredoxin with a known x-ray crystal structure, which includes the first treatment of net atomic charges in the iron-sulfur clusters, was determined using a macroscopic electrostatic model.