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Redox enzyme engineering: conversion of human glutathione reductase into a trypanothione reductase

โœ Scribed by Bradley, Mark; Buecheler, Uwe S.; Walsh, Christopher T.


Book ID
123617291
Publisher
American Chemical Society
Year
1991
Tongue
English
Weight
490 KB
Volume
30
Category
Article
ISSN
0006-2960

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Redox interconversion of glutathione red
โœ Ana M. Mata; M. Carmen Pinto; Juan Lรณpez-Barea ๐Ÿ“‚ Article ๐Ÿ“… 1985 ๐Ÿ› Springer ๐ŸŒ English โš– 977 KB

The glutathione reductase from E. coli was rapidly inactivated following aerobic incubation of the pure and cell-free extract enzymes with NADPH, NADH and other reductants. The inactivation of the pure enzyme depended on the time and temperature of incubation (t 1/2 = 2 min at 37 degrees C), and was