✦ LIBER ✦

Redox changes in coenzyme Q in the millisecond time range: An approach using rapid quenching and high-performance liquid chromatography

✍ Scribed by Ah-Lim Tsai; Robert Kauten; Graham Palmer

Publisher: Elsevier Science
Year: 1985
Tongue: English
Weight: 434 KB
Volume: 151
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(85)90062-4

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✦ Synopsis

We have combined a rapid-quenching protocol with HPLC analysis to measure the kinetics of reduction of coenzyme Q in a mitochondrial enzyme complex. The method has a time resolution of several milliseconds and will readily measure 1-20 nmol of the Q derivatives under investigation. By changing the HPLC solvent, either Q6 or Q10 can be studied.