Redox and structural aspects on iron inositol 1,2,3-trisphosphate interaction: An experimental and computational approach
✍ Scribed by Nicolás Veiga; Julia Torres; Fernanda Cerdá; Gabriel González; Kerman Gómez; David Mansell; Sally Freeman; Sixto Domínguez; Carlos Kremer
- Publisher
- Elsevier Science
- Year
- 2011
- Tongue
- English
- Weight
- 786 KB
- Volume
- 994
- Category
- Article
- ISSN
- 0022-2860
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✦ Synopsis
Among small cellular molecules, certain inositol phosphates (InsPs) excel at complexing Fe 3+ in a safe manner in vitro. In particular Ins(1,2,3)P 3 is probably an important chelator of iron cations not strongly bound to proteins, which avoids iron redox cycling and the associated production of free radicals. In this work we report the first thermodynamic data of the Fe 3+ /Fe 2+ redox potential in the presence of Ins(1,2,3)P 3 which demonstrate the capability of this ligand to inhibit iron(III) reduction in the intracellular media. The Fe 3+ -Ins(1,2,3)P 3 speciation was also studied by 31 P NMR measurements, focusing on the structure of the complexes. The work is completed by theoretical calculations which lead us to predict the geometries of the species at different pH values and their relative stabilities. The influence of the ring conformation in the Fe 3+ complexed forms is also discussed.