Red wine proteins: Two dimensional (2-D) electrophoresis and mass spectrometry analysis
โ Scribed by Mainente, Federica; Zoccatelli, Gianni; Lorenzini, Marilinda; Cecconi, Daniela; Vincenzi, Simone; Rizzi, Corrado; Simonato, Barbara
- Book ID
- 124122601
- Publisher
- Elsevier Science
- Year
- 2014
- Tongue
- English
- Weight
- 542 KB
- Volume
- 164
- Category
- Article
- ISSN
- 0308-8146
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๐ SIMILAR VOLUMES
Two-dimensional polyacrylamide gel electrophoresis and mass spectrometry is a powerful combination for the separation of complex protein mixtures in biological samples and the subsequent identification of individual polypeptides. We have used this approach to construct a database of proteins of the
Capillary electrophoresis/time-of-flight mass spectrometry(CE/TOFMS) has been used for analysis of in-gel digests of protein spots excised from two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (2-D SDS-PAGE). An off-line purification and preconcentration procedure with a Zip