Red Blood Cell Templated Polyelectrolyte Capsules: A Novel Vehicle for the Stable Encapsulation of DNA and Proteins
✍ Scribed by Oliver Kreft; Radostina Georgieva; Hans Bäumler; Martin Steup; Bernd Müller-Röber; Gleb B. Sukhorukov; Helmuth Möhwald
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 263 KB
- Volume
- 27
- Category
- Article
- ISSN
- 1022-1336
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Summary: A novel method for the encapsulation of biomacromolecules, such as nucleic acids and proteins, into polyelectrolyte microcapsules is described. Fluorescence‐labelled double‐stranded DNA and human serum albumin (HSA) are used as model substances for encapsulation in hollow microcapsules templated on human erythrocytes. The encapsulation procedure involves an intermediate drying step. The accumulation of DNA and HSA in the capsules is observed by confocal laser scanning microscopy, UV spectroscopy, and fluorimetry. The mechanism of encapsulation is discussed.
Confocal fluorescence microscopy images of encapsulated TRITC‐HSA (left) and dsDNA (right). Inserts demonstrate fluorescence profiles for both compounds.
magnified imageConfocal fluorescence microscopy images of encapsulated TRITC‐HSA (left) and dsDNA (right). Inserts demonstrate fluorescence profiles for both compounds.