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Recycling of NAD+using coimmobilized alcohol dehydrogenase andE. coli

✍ Scribed by C. Burstein; H. Ounissi; M. D. Legoy; G. Gellf; D. Thomas

Book ID
112896136
Publisher
Springer-Verlag
Year
1981
Tongue
English
Weight
469 KB
Volume
6
Category
Article
ISSN
0273-2289

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Selective enzyme amplification of NAD+/N
Selective enzyme amplification of NAD+/NADH using coimmobilized glycerol dehydrogenase and diaphorase with amperometric detection
✍ Karin Kronkvist; Katarina Wallentin; Gillis Johansson πŸ“‚ Article πŸ“… 1994 πŸ› Elsevier Science 🌐 English βš– 731 KB

A flow system for substrate recycling of NAD+/NADH was set up with an enzyme reactor containing coimmobiliied glycerol dehydrogenase (GDH) and diaphorase. The product from the diaphorase catalysis, hexacyanoferrate(II), was detected amperometrically at a glassy carbon electrode. The amplification fa