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Recurrent allelic deletions of chromosome arms 15q and 16q in human small cell lung carcinomas

✍ Scribed by Sasha E. Stanton; Sang Won Shin; Bruce E. Johnson; Matthew Meyerson

Publisher: John Wiley and Sons
Year: 2000
Tongue: English
Weight: 102 KB
Volume: 27
Category: Article
ISSN: 1045-2257
DOI: 10.1002/(sici)1098-2264(200003)27:3<323::aid-gcc14>3.0.co;2-p

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✦ Synopsis

The genetic lesions that lead to the development of small cell lung carcinoma (SCLC) remain incompletely defined. To identify recurrent allelic deletions in specific chromosomal regions that could serve as markers for tumor suppressor gene (TSG) inactivation in SCLC, we performed a comprehensive allelotype analysis of all 39 nonacrocentric autosomal arms. Alterations in 158 polymorphic microsatellite alleles were examined in 24 pairs of human SCLC tumor and normal control DNA samples. A total of 2,107 informative reactions were analyzed. This analysis revealed allelic losses of 100% on chromosome arm 3p, Ͼ85% loss within chromosome arms 13q and 17p, and Ͼ70% loss within chromosome arms 4q, 5q, 15q, and 16q. The allelic deletions on chromosome arms 15q and 16q have not been defined previously for SCLC and are candidate regions to harbor novel TSGs.

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