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Recruitment of enzyme activity in albumin by molecular imprinting

✍ Scribed by Yuichi Ohya; Junya Miyaoka; Tatsuro Ouchi


Book ID
102490275
Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
213 KB
Volume
17
Category
Article
ISSN
1022-1336

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✦ Synopsis


Abstract

The catalytic activity of bovine serum albumin (BSA) modified physically by molecular imprinting using transition‐state analogue (TSA) as a template molecule was studied. The resultant imprinted serum albumin (Imp‐BSA) showed the rate acceleration of dehydrofluorination reaction from (4__R__,4__S__)‐4‐fluoro‐4‐(4‐nitrophenyl)butan‐2‐one (1) and followed the type of Michaelis‐Menten reaction in ethyl acetate solution. The enzymatic activity of Imp‐BSA was competitively inhibited by (4__R__,4__S__)‐4‐hydroxy‐4‐(4‐nitrophenyl)butan‐2‐one (4).


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