## Abstract A novel molecularly imprinted hydrogel for bovine serum albumin (BSA) was prepared using cupric ion as the bridge between the template BSA and the functional monomer 4‐vinylpyridine. N‐Isopropylacrylamide (NIPA) was used as an assistant monomer to provide the stimuli‐responsibility of t
Recruitment of enzyme activity in albumin by molecular imprinting
✍ Scribed by Yuichi Ohya; Junya Miyaoka; Tatsuro Ouchi
- Book ID
- 102490275
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 213 KB
- Volume
- 17
- Category
- Article
- ISSN
- 1022-1336
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✦ Synopsis
Abstract
The catalytic activity of bovine serum albumin (BSA) modified physically by molecular imprinting using transition‐state analogue (TSA) as a template molecule was studied. The resultant imprinted serum albumin (Imp‐BSA) showed the rate acceleration of dehydrofluorination reaction from (4__R__,4__S__)‐4‐fluoro‐4‐(4‐nitrophenyl)butan‐2‐one (1) and followed the type of Michaelis‐Menten reaction in ethyl acetate solution. The enzymatic activity of Imp‐BSA was competitively inhibited by (4__R__,4__S__)‐4‐hydroxy‐4‐(4‐nitrophenyl)butan‐2‐one (4).
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