Recovery of proteins and other biological compounds using fibrous materials: I. Adsorption by salt addition

✍ Scribed by Li Ang Chen; Ruben G Carbonell; George A Serad

• Publisher: Wiley (John Wiley & Sons)
• Year: 1999
• Tongue: English
• Weight: 196 KB
• Volume: 74
• Category: Article
• ISSN: 0268-2575
• DOI: 10.1002/(sici)1097-4660(199908)74:8<733::aid-jctb111>3.0.co;2-4

✦ Synopsis

An adsorption and ®ltration process for the recovery of proteins and other biological compounds from aqueous streams has been developed, using cellulose-based ®brous materials. Of the many cellulose derivatives studied, cellulose acetate ®brets (CAF) and cellulose triacetate ®brets (CTF) have been shown to be the most effective. In the presence of salts, they lead to protein adsorption by hydrophobic interactions. Model proteins, such as bovine serum albumin (BSA), have been recovered by incubating these solutions with CTF in the presence of ammonium sulfate, followed by ®ltration through a 20 mm pore size ®lter. The amount of salt necessary varies with the protein type, but decreases with increasing temperature and protein concentration. High protein recovery has been obtained from an actual wastewater system at low salt dosages.