✦ LIBER ✦
Recovery of nanogram quantities of DNA from plasma and quantitative measurement using labeling by nick translation
✍ Scribed by Gilbert J. Fournié; Martine Gayral-Taminh; Jean-Pierre Bouché; Jean J. Conté
- Publisher
- Elsevier Science
- Year
- 1986
- Tongue
- English
- Weight
- 622 KB
- Volume
- 158
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
A method which allows for the quantitative measurement of DNA in plasma is described. After treatment of plasma with phenol, DNA is precipitated by ethanol using gelatin as a coprecipitating agent. DNA is then measured by nick translation labeling. This assay takes a few hours. It is suitable for the measurement of DNA within a range of 0.02 to 20 ng in 10 ~1 of plasma. For example, it is applied to the measurement of DNA in plasma from mice injected with bacterial lipopolysaccharide. 0 1986 Academic PES, 1~.