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Recovery of lymphocytes from clotted blood for HLA typing

✍ Scribed by I.S. Cox; B. Lichtiger; J.P. Holmes


Book ID
118897306
Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
182 KB
Volume
22
Category
Article
ISSN
0022-1759

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The amount of adenosine triphosphate (ATP) in human lymphocytes was determined using a technique based on light emission from a bioluminescent reaction with luciferin-luciferase. The amount of ATP changed when cells were incubated in the presence of specific HLA antisera and complement. For determin