Recovery of cells in vitro from the effects of hypoxia and hyperoxia

✍ Scribed by David H. Shaw; Donald M. Pace

• Publisher: John Wiley and Sons
• Year: 1969
• Tongue: English
• Weight: 427 KB
• Volume: 73
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1040730205

✦ Synopsis

The cytotoxic effect of high, as well as low, oxygen tension of proliferation and metabolism of Low line cells in culture is reversible even after several days of exposure provided the cells are returned to 95% air + 5% COZ environment. This suggests that the activity of certain mechanisms within the cells may have been altered or in other ways inhibited by the abnormal environments but are quite rapidly regenerated once the adverse condition is removed. The cells tolerate a low 0 2 exposure for at least 20 days while continuous exposure to high 0 2 atmosphere results in degeneration and death after 7-10 days. Both glucose utilization and lactic acid production are elevated in cultures exposed to either low or high 0 2 tensions, although they are markedly higher in the latter condition. When cell so exposed are returned to a n air + 5% COZ atmosphere, rate of glucose uptake and lactic acid formation soon approaches that found in control cultures.