Recovery of bacterial spores and vegetative cells with a miniature spray drier
✍ Scribed by Rhian, Morris ;Lynch, Daniel C.
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1961
- Weight
- 341 KB
- Volume
- 3
- Category
- Article
- ISSN
- 0368-1467
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
A laboratory spray drier with a gas flow of 2 to 3 ft^3^/min was used to dry suspensions containing spores of Bacillus anthracis or cells of Pasteurella tularensis. Dried material was collected in a 1/2‐in. diameter cyclone that retained about 97 per cent of the product from the drier. The particle size distributions of the powders varied with the nature of substances in the suspension to be dried; as much as 80 per cent of the mass in particles 5 μ or less in diameter was obtained only when egg yolk was added to the suspensions. Retention of viability was about 90 per cent for B. anthracis spores and these powders contained from 175 to 522 × 10^9^ viable spores/gram. Retention of viability of P. tularensis cells was 0·6 per cent or greater only when the suspension contained a relatively high proportion of stabilizers. With sucrose‐skim milk‐thiourea stabilizer about 10 per cent recovery of viability was obtained when the proportion of stabilizer solids to other solids was 3 : 1 or 6 : 1. These powders contained 140 × 10^9^ viable cells per gram and the mass median diameter was 6·0 μ. The properties of the powders suggest that spray dried vaccines may be prepared for use either in suspension or in aerosols.