Recovery of a monoclonal antibody from hybridoma culture supernatant by affinity precipitation with Eudragit S-100

Preliminary studies on the purification

Preliminary studies on the purification of a monoclonal antibody by affinity precipitation with Eudragit S-100

✍ M. Angela Taipa; Rajni Kaul; Bo Mattiasson; Joaquim M. S. Cabral 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 53 KB 👁 2 views

A simple procedure for the purification of an IgG-type monoclonal antibody by affinity precipitation using Eudragit S-100 is presented. The ligand, a microbial lipase previously used as antigen, was coupled to the polymer at a concentration of 40 mg lipase/g Eudragit. This macroligand was reversibly