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Recovery and characterization ofBalanites aegyptiacaDel. kernel proteins. Effect of defatting, air classification, wet sieving and aqueous ethanol treatment on solubility, digestibility, amino acid composition and sapogenin content

✍ Scribed by Mohamed, A. M. ;Wolf, W. ;Spiess, W. E. L.


Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
81 KB
Volume
44
Category
Article
ISSN
0027-769X

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✦ Synopsis


In order to find alternative protein sources in African regions where protein deficiency in nutrition is prevailing, solubility, in-vitro digestibility, amino acid composition and chemical score of Balanites aegyptiaca Del. kernel proteins were investigated as a function of different processing steps including defatting, air classification, wet sieving and aqueous ethanol treatment. Air classification delivered a fine fraction of 58.1% of the total protein. Applying a wet sieving process, a protein concentrate of 72.9% protein content was achieved but the recovery was very low (35.6%). However, in case of isoelectric precipitation followed by aqueous ethanol treatment both protein content (78.2%) and recovery (53.7%) were high. Data concerning the chemical score revealed, that lysine content of the defatted kernel flour amounted to 74.2% of the recommended FAO/WHO standard level. In-vitro protein digestibility was found to be higher than of legume proteins. The digestible protein of the full fat flour, defatted flour, air classified and wet sieved fine fractions and protein concentrate were 91.9, 93.7, 82.0, 86.4 and 94.2%, respectively. The sapogenin content per 100 g protein of the investigated protein preparations was significantly lower (46% to 62%) than of the initial material (oilcake).