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Recovering and reamplifying of the differentially expressed cDNA bands isolated from mRNA differential display

โœ Scribed by Hong Wang; Zhenbiao Xia; Yaohe Wang; Chunhai Li; Li Ya Sun


Book ID
110671125
Publisher
Humana Press Inc
Year
1998
Tongue
English
Weight
232 KB
Volume
9
Category
Article
ISSN
1073-6085

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We have developed a novel efficient approach, termed differential subtraction display, for the identification of differentially expressed genes. Several critical parameters for the reproducibility and enhanced sensitivity of display, as well as steps to reduce the number of false positive cDNA speci