A method is described that allows a three-dimensional object to be reconstructed from micrographs of serial sections by means of computer graphics. The reconstructed object, which can be rotated three-dimensionally, is displayed on a colour visual display unit, and the object is shaded in order to p
Reconstruction using photographed serial sections
โ Scribed by Berns, Michael W.
- Publisher
- John Wiley and Sons
- Year
- 1967
- Tongue
- English
- Weight
- 285 KB
- Volume
- 159
- Category
- Article
- ISSN
- 0003-276X
No coin nor oath required. For personal study only.
โฆ Synopsis
A method is described whereby serial sections are photographed through a microscope and printed on a clear acetate film. The pictures are mounted on Plexiglas rectangles and placed back to back. A solid stack of plates with the reconstructed structure visible inside, or a slide-box arrangement of every other section may be produced. Variations of the technique are presented so that one can print high-contrast or low-contrast pictures, reconstruct simple or complex structures, and work more rapidly with less equipment. This is a simple, accurate, and rapid method.
๐ SIMILAR VOLUMES
Background: Computer-based, three-dimensional reconstruction of histological sections is necessary for solving a diversity of questions in morphology and anatomy. Programming software for this purpose is difficult and time-consuming. Therefore, we tested if commercially available product-design soft