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Reconstitution Reveals Additional Roles for N- and C-Terminal Domains of Gα in Muscarinic Receptor Coupling †

✍ Scribed by Slessareva, Janna E.; Graber, Stephen G.


Book ID
126842073
Publisher
American Chemical Society
Year
2003
Tongue
English
Weight
113 KB
Volume
42
Category
Article
ISSN
0006-2960

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## Abstract Transmembrane domains (TMDs) of G‐protein coupled receptors (GPCRs) have very low water solubility and often aggregate during purification and biophysical investigations. To circumvent this problem many laboratories add oligolysines to the __N__‐ and __C__‐termini of peptides that corre