๐”– Bobbio Scriptorium
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Reconstitution of the low density lipoprotein receptor

โœ Scribed by Wolfgang J. Schneider

Publisher
John Wiley and Sons
Year
1983
Tongue
English
Weight
767 KB
Volume
23
Category
Article
ISSN
0730-2312

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โœฆ Synopsis

The receptor for low density lipoprotein was purified from bovine adrenal cortex in the presence of the nonionic detergent octylglucoside. Receptors were incorporated into the bilayer of egg phosphatidylcholine vesicles by a detergent-dialysis method. Reconstituted receptors were functional in that they bound low density lipoprotein as well as a monoclonal antibody directed against the receptor in a specific, saturable fashion. Binding activity of reconstituted receptors was measured by a gel chromatography assay. The orientation of the receptor molecule within the phospholipid bilayer was investigated by binding assays following proteolytic digestion. Reconstituted receptors showed an orientation that was functionally indistinguishable from that of low density lipoprotein receptors in the plasma membrane of intact human fibroblasts.

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