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Reconstitution of the glucose transport activity of rat adipocytes

โœ Scribed by Diana Mark Malchoff; Virginia G. Parker; Robert G. Langdon

Book ID
115726913
Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
957 KB
Volume
817
Category
Article
ISSN
0005-2736

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An improved method of reconstitution of
An improved method of reconstitution of adipocyte glucose transport activity
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The glucose transport activity of rat epididymal fat cells was reconstituted into egg lecithin liposomes with a high degree of reproducibility. The activity was solubilized with 20 mM sodium cholate in Buffer B (10 mM Tris-HCl, pH 7.5). After elimination of small molecules by gel filtration, the tra