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Reconstitution of cytochrome c oxidase into phospholipid vesicles: Effect of detergents

✍ Scribed by Beatrice Vallone; Emilio D'Itri; Giovanni Antonini; Francesco Malatesta; Paolo Sarti

Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
410 KB
Volume
23
Category
Article
ISSN
1567-5394

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A method for the rapid incorporation of cytochrome c oxidase into membranes has been developed. This method essentially consists of obtaining a preparation of the enzyme in which it is isolated and then dissolving it in a medium containing 0.5% of the detergent Tween 20, which gives a final concentr