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Reconstitution of Cardiac Sarcoplasmic Reticulum Calcium Channels

✍ Scribed by ALAN J. WILLIAMS; RICHARD H. ASHLEY


Book ID
119863293
Publisher
John Wiley and Sons
Year
1989
Tongue
English
Weight
503 KB
Volume
560
Category
Article
ISSN
0890-6564

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In order to study the conductances of the Sarcoplasmic Reticulum (SR) membrane, microsomal fractions from cardiac SR were isolated by differential and sucrose gradient centrifugations and fused into planar lipid bilayers (PLB) made of phospholipids. Using either KCl or K-gluconate solutions, a large