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Reconstitution into liposomes of the Na+-dependent transport systems for cyclacillin, an aminopenicillin, from brush border membranes of rat small intestine

✍ Scribed by Toshikiro Kimura; Hiroyuki Kobayashi; Hitoshi Sezaki

Book ID
118940040
Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
324 KB
Volume
27
Category
Article
ISSN
0024-3205

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High-affinity phlorizin binding to brush
High-affinity phlorizin binding to brush border membranes from small intestine: Identity with (a part of) the glucose transport system, dependence on the Na+-gradient, partial purification
✍ Tannenbaum, Carl ;Toggenburger, Gerhard ;Kessler, Markus ;Rothstein, Aser ;Semen πŸ“‚ Article πŸ“… 1977 πŸ› Wiley (John Wiley & Sons) 🌐 English βš– 794 KB

In the presence of an NaSCN gradient phlorizin binds with a high affinity (Kd 4.7 pM) t o vesicles derived from brush border membranes of intestinal cells of rabbits. The value for Kd corresponds closely t o that of Ki determined from phlorizin inhibition of sugar transport. The apparent affinity fo