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Reconstitution and Purification of Eukaryotic Initiation Factor 2B (eIF2B) Expressed in Sf21 Insect Cells

✍ Scribed by John R. Fabian; Scot R. Kimball; Leonard S. Jefferson


Book ID
115645297
Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
389 KB
Volume
13
Category
Article
ISSN
1046-5928

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The functional regulation of the human bradykinin B2 receptor expressed in sf21 cells was studied. Human bradykinin B2 receptors were immunodetected as a band of 75-80 kDa in membranes from recombinant baculovirus-infected cells and visualized at the plasma membrane, by confocal microscopy, using an