Reconstituting the Cytoskeleton

✍ Scribed by Ronald D. Vale (Eds.)

• Publisher: Academic Press
• Year: 2014
• Tongue: English
• Leaves: 480
• Series: Methods in Enzymology 540
• Edition: 1
• Category: Library

✦ Synopsis

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers cytoskeletal structure, including such topics as rotational movement of formins studied by fluorescence polarization microscopy, in vitro assembly assays for bacterial actin filaments, and modulators of microtubule plus end growth.

• Continues the legacy of this premier serial with quality chapters authored by leaders in the field
• Covers cytoskeletal structure
• Contains chapters with such topics as reconstitution of organelle transport and assaying microtubule nucleation by gamma-tubulin ring complex.

✦ Table of Contents

Content:
Series Page
Page ii

Copyright
Page iv

Contributors
Pages xiii-xviii

Preface: The Role of Reconstitution in Cytoskeleton Research
Pages xix-xxiii
Ronald D. Vale

Chapter One - Actin Filament Dynamics Using Microfluidics
Pages 3-17
Marie-France Carlier, Guillaume Romet-Lemonne, Antoine Jégou

Chapter Two - Bacterial Actin-Like Proteins: Purification and Characterization of Self-Assembly Properties
Pages 19-34
Natalie A. Petek, R. Dyche Mullins

Chapter Three - Quantitative Analysis of Microtubule Self-assembly Kinetics and Tip Structure
Pages 35-52
Louis S. Prahl, Brian T. Castle, Melissa K. Gardner, David J. Odde

Chapter Four - Biochemical Reconstitution of the WAVE Regulatory Complex
Pages 55-72
Baoyu Chen, Shae B. Padrick, Lisa Henry, Michael K. Rosen

Chapter Five - Rotational Movement of Formins Evaluated by Using Single-Molecule Fluorescence Polarization
Pages 73-94
Hiroaki Mizuno, Naoki Watanabe

Chapter Six - Single-Molecule Studies of Actin Assembly and Disassembly Factors
Pages 95-117
Benjamin A. Smith, Jeff Gelles, Bruce L. Goode

Chapter Seven - Assaying Microtubule Nucleation by the γ-Tubulin Ring Complex
Pages 119-130
Yuk-Kwan Choi, Robert Z. Qi

Chapter Eight - Reconstituting Dynamic Microtubule Polymerization Regulation by TOG Domain Proteins
Pages 131-148
Jawdat Al-Bassam

Chapter Nine - Generation of Differentially Modified Microtubules Using In Vitro Enzymatic Approaches
Pages 149-166
Annapurna Vemu, Christopher P. Garnham, Duck-Yeon Lee, Antonina Roll-Mecak

Chapter Ten - Engineering Defined Motor Ensembles with DNA Origami
Pages 169-188
Brian S. Goodman, Samara L. Reck-Peterson

Chapter Eleven - Construction and Analyses of Elastically Coupled Multiple-Motor Systems
Pages 189-204
Arthur Rogers, Pamela E. Constantinou, D. Kenneth Jamison, Jonathan W. Driver, Michael R. Diehl

Chapter Twelve - Reconstitution of Cortical Dynein Function
Pages 205-230
Sophie Roth, Liedewij Laan, Marileen Dogterom

Chapter Thirteen - Reconstitution of Microtubule-Dependent Organelle Transport
Pages 231-248
Pradeep Barak, Ashim Rai, Alok Kumar Dubey, Priyanka Rai, Roop Mallik

Chapter Fourteen - Reconstituting the Motility of Isolated Intracellular Cargoes
Pages 249-262
Adam G. Hendricks, Yale E. Goldman, Erika L.F. Holzbaur

Chapter Fifteen - Reconstitution of Contractile Actomyosin Arrays
Pages 265-282
Michael Murrell, Todd Thoresen, Margaret Gardel

Chapter Sixteen - Directed Actin Assembly and Motility
Pages 283-300
Rajaa Boujemaa-Paterski, Rémi Galland, Cristian Suarez, Christophe Guérin, Manuel Théry, Laurent Blanchoin

Chapter Seventeen - In Vitro Reconstitution of Dynamic Microtubules Interacting with Actin Filament Networks
Pages 301-320
Magdalena Preciado López, Florian Huber, Ilya Grigoriev, Michel O. Steinmetz, Anna Akhmanova, Marileen Dogterom, Gijsje H. Koenderink

Chapter Eighteen - Measuring Kinetochore–Microtubule Interaction In Vitro
Pages 321-337
Jonathan W. Driver, Andrew F. Powers, Krishna K. Sarangapani, Sue Biggins, Charles L. Asbury

Chapter Nineteen - Micropattern-Guided Assembly of Overlapping Pairs of Dynamic Microtubules
Pages 339-360
Franck J. Fourniol, Tai-De Li, Peter Bieling, R. Dyche Mullins, Daniel A. Fletcher, Thomas Surrey

Chapter Twenty - WAVE Regulatory Complex Activation
Pages 363-379
Peter J. Hume, Daniel Humphreys, Vassilis Koronakis

Chapter Twenty-One - Dissecting Principles Governing Actin Assembly Using Yeast Extracts
Pages 381-397
Alphée Michelot, David G. Drubin

Chapter Twenty-Two - Xenopus Egg Cytoplasm with Intact Actin
Pages 399-415
Christine M. Field, Phuong A. Nguyen, Keisuke Ishihara, Aaron C. Groen, Timothy J. Mitchison

Chapter Twenty-Three - Glycogen-Supplemented Mitotic Cytosol for Analyzing Xenopus Egg Microtubule Organization
Pages 417-433
Aaron C. Groen, Phuong A. Ngyuen, Christine M. Field, Keisuke Ishihara, Timothy J. Mitchison

Chapter Twenty-Four - Spindle Assembly on Immobilized Chromatin Micropatterns
Pages 435-448
Céline Pugieux, Serge Dmitrieff, Katarzyna Tarnawska, François Nédélec

Author Index
Pages 449-470

Subject Index
Pages 471-484