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Recombination of human erythrocyte apoprotein and lipid. II. Visualization of apoprotein-lipid bilayer complex by freeze-etching

✍ Scribed by Wehrli, Ernst ;Morse, Philip D.

Book ID: 102924387
Publisher: Wiley (John Wiley & Sons)
Year: 1974
Tongue: English
Weight: 841 KB
Volume: 2
Category: Article
ISSN: 0091-7419
DOI: 10.1002/jss.400020108

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✦ Synopsis

Abstract

Bilayers of human erythrocyte apoprotein‐lipid complexes were made by dipping a mica plate through monolayers of the complex formed at the air‐water interface. Stearic acid and erythrocyte lipid alone served as controls. Freeze‐fracture images of the complex at high lipid surface pressures (30 dynes/cm) showed particles (average diameter, 109 Å ± 18 Å) similar to those of erythrocyte ghosts (average diameter, 102 Å ± 19 Å). Control surfaces were smooth. We conclude that part or all of the protein molecule penetrated into the lipid bilayer and that erythrocyte apoprotein‐lipid complexes yield fracture faces similar to the native erythrocyte membrane.

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Recombination of human erythrocyte apoprotein and lipid. I. Interaction of apoprotein and lipid at the air-water interface

✍ Morse, Philip D. 📂 Article 📅 1974 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 496 KB

## Abstract The formation and stabilization of a complex between total erythrocyte apoprotein and monolayers of total erythrocyte lipid as measured by changes of surface pressure (Δπ) and rate of change of surface pressure (dπ/dt) was studied as a function of pH, ionic strength, and lipid surface p