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Recombination between repeats in Escherichia coli by a recA-independent, proximity-sensitive mechanism

โœ Scribed by Lovett, S. T. ;Gluckman, T. J. ;Simon, P. J. ;Sutera, V. A. ;Drapkin, P. T.

Publisher
Springer
Year
1994
Tongue
English
Weight
864 KB
Volume
245
Category
Article
ISSN
0026-8925

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โœ Miura-Masuda, Akiko ;Ikeda, Hideo ๐Ÿ“‚ Article ๐Ÿ“… 1990 ๐Ÿ› Springer ๐ŸŒ English โš– 855 KB

A system for detecting a spontaneous deletion in Escherichia coli was developed and the role of DNA gyrase in deletion formation was studied. A derivative of 2plac5, 2AM36, was isolated in which whole pBR322 DNA was inserted in the lacZ gene and 227 bp of the lac gene duplicated at both sides of the