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Recombinant tissue plasminogen activator reduces incidence of catheter malfunction and bacteremia

✍ Scribed by Lilit A Sargsyan; Risheng Xu; Alicia Romero; Brett W. Stephens; Donald A Molony


Publisher
Wiley (John Wiley & Sons)
Year
2011
Tongue
English
Weight
71 KB
Volume
40
Category
Article
ISSN
0090-2934

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Stable transformation was used to generate a cloned insect cell line (Bm5 silkmoth cells) overexpressing human tissue plasminogen activator (tPA). This cell line expressed 135 Β΅g/mL single chain tPA in serum-free medium in static culture with a maximum specific activity of 120 IU/Β΅g. In serum-contai