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Recombinant RNA polymerase: inducible overexpression, purification and assembly of Escherichia coli rpo gene products

โœ Scribed by Zalenskaya, Katya; Lee, Jookyung; Gujuluva, Chandrasekhar N.; Shin, Yong Ki; Slutsky, Maya; Goldfarb, Alex


Book ID
122857483
Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
718 KB
Volume
89
Category
Article
ISSN
0378-1119

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## Abstract The biochemical characterization of the bacterial transcription cycle has been greatly facilitated by the production and characterization of targeted RNA polymerase (RNAP) mutants. Traditionally, RNAP preparations containing mutant subunits have been produced by reconstitution of denatu