β Scribed by Charles Cunningham, Andrew J. R. Porter
No coin nor oath required. For personal study only.
Univ. of Aberdeen, Scotland. Text providing comprehensive and detailed protocols covering all the latest molecular approaches. For new and established reseachers and biotechnoloists.
Cover......Page 1
Contents......Page 2
1. Rice Transformation by Agrobacterium Infection......Page 13
2. Barley as a Producer of Heterologous Protein......Page 22
3. Introducing and Expressing Genes in Legumes......Page 37
4. Use of the GUS Reporter Gene......Page 49
5. Expression of Recombinant Proteinase Inhibitors in Plants......Page 58
6. Detection of Recombinant Viral Coat Protein in Transgenic Plants......Page 74
7. Synthesis of Recombinant Human Cytokine GM-CSF in the Seeds of Transgenic Tobacco Plants......Page 85
8. Tobamovirus Vectors for Expression of Recombinant Genes in Plants......Page 96
9. Single-Chain Fv Antibodies Expressed in Plants......Page 109
10. Characterization and Applications of Plant-Derived Recombinant Antibodies......Page 134
11. Production of Recombinant Antibodies in Plant Suspension Cultures......Page 148
12. Production of Foreign Proteins in Tobacco Cell Suspension Culture......Page 160
13. Transient Gene Expression in Plant Protoplasts......Page 170
14. Stability of Recombinant Proteins in Plants......Page 181
15. Trafficking and Stability of Heterologous Proteins in Transgenic Plants......Page 193
16. Screening for Transgenic Lines with Stable and Suitable Accumulation Levels of a Heterologous Protein......Page 207
17. Manipulation of Photosynthetic Metabolism......Page 232
18. Quantification of Heterologous Protein Levels in Transgenic Plants by ELISA......Page 253
19. Analysis of N - and O -Glycosylation of Plant Proteins......Page 272
20. Two-Dimensional Electrophoresis......Page 292
Index......Page 306
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