Recombinant protein production by Mut+ strain of Pichia pastoris using dual carbon sources: methanol and sorbitol

✍ Scribed by P. Calik; E. Celik; S.G. Oliver

• Publisher: Elsevier
• Year: 2009
• Tongue: English
• Weight: 86 KB
• Volume: 25
• Category: Article
• ISSN: 1871-6784
• DOI: 10.1016/j.nbt.2009.06.286

✦ Synopsis

In the studies involving other microorganisms it was shown that addition of carboxylic acids with carbon chains containing five and more carbons as substrate can induce incorporation of the respective ␤-hydroxy acids into the PHA. In our experiments luminous bacteria were grown on media supplemented with valeric acid. PHAs synthesized by the most of studied strains of P. leiognathi contained significantly larger amounts of ␤-hydroxyvaleric acid in such conditions. It was shown that V. fisheri, strain 2089, included up to 1.5% ␤-hydroxyvalerate in PHAs. For V. harveyi influence of valeric acid on polymer composition was not detected. Also, no minor hydroxy acids were found in the PHAs produced by P. phosphoreum regardless of the addition of valerate.

Thus, P. leiognathi and V. fisheri cells grown in the medium supplemented with valerate, which is hydroxyvalerate precursor substrate, can produce polymers containing increased amounts of this hydroxy acid. By contrast, P. phosphoreum strains were found unable to synthesize multi-component PHAs.