Recombinant production and purification of the subunit c of chloroplast ATP synthase
β Scribed by Robert M. Lawrence; Benjamin Varco-Merth; Christopher J. Bley; Julian J.-L. Chen; Petra Fromme
- Book ID
- 116829246
- Publisher
- Elsevier Science
- Year
- 2011
- Tongue
- English
- Weight
- 887 KB
- Volume
- 76
- Category
- Article
- ISSN
- 1046-5928
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A cDNA clone encoding the complete precursor of the gamma subunit of the pea chloroplast ATP synthase has been isolated from a pea leaf cDNA library in lambda gt 11 following detection with antibodies to the purified gamma subunit. The cDNA insert of 1.4 kbp is smaller than transcripts of about 1.6
Three CFt and three CF 0 subunits of the chloroplast H+-ATP synthase are encoded on the chloroplast genome. The chloroplast atp genes are organized as two operons in plants but not in the green alga, Chlamydomonas reinhardtii. The atpBE or /~ operon shows a relatively simple organisation and transcr