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Recombinant human insulin IX. Investigation of factors, influencing the folding of fusion protein-S-sulfonates, biotechnological precursors of human insulin

✍ Scribed by Roman V Tikhonov; Sergey E Pechenov; Irina A Belacheu; Sergey A Yakimov; Vadim E Klyushnichenko; Heloisa Tunes; Josef E Thiemann; Luciano Vilela; Andrey N Wulfson


Book ID
117702545
Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
343 KB
Volume
26
Category
Article
ISSN
1046-5928

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The application of gene fusion technology for the production of heterologous proteins in Escherichia coli has required the development of specific cleavage methods to separate the coexpressed fusion protein partner from the protein of interest. When hydroxylamine is used to cleave Asn-Gly fusion pro