When grown on a medium containing 5 g maltodextrin L -1 , Aspergillus niger transformant N402[pAB6-10]B1, which has an additional 20 copies of the glucoamylase (glaA) gene, produced 320 ยฑ 8 mg (mean ยฑ S.E.) glucoamylase (GAM) L -1 in batch culture and 373 ยฑ 9 mg GAM L -1 in maltodextrin-limited chem
Recombinant Glucoamylase Production byAspergillus nigerB1 in Chemostat and pH Auxostat Cultures
โ Scribed by Richard J. Swift; Marilyn G. Wiebe; Geoffrey D. Robson; Anthony P.J. Trinci
- Book ID
- 115608993
- Publisher
- Elsevier Science
- Year
- 1998
- Tongue
- English
- Weight
- 218 KB
- Volume
- 25
- Category
- Article
- ISSN
- 1087-1845
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Fusarium venenatum (formerly Fusarium graminearum) JeRS 325 produces heterologous glu- coamylase (GAM) under the regulation of a Fusarium oxysporum alkaline (trypsin-like) protease promoter. The glucoamylase gene was used as a reporter gene to study the effects of ammonium and pH on GAM production u