Recombinant f1 Phage-Mediated Transfection of Mammalian Cells Using Lipopolyamine Technique
✍ Scribed by M. Yokoyamakobayashi; S. Kato
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 417 KB
- Volume
- 223
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
Recombinant fl phages carrying a shuttle vector pKA1M for expression of blasticidin (S) deaminase were introduced into monkey (\operatorname{COS} 7) cells by mixing with dioctadecylamidoglycylspermine (DOGS). Blasticidin S selection resulted in the detectable growth of resistant colonies within a week. The transfection efficiency depended on the amounts of the phage and DOGS, their ratio, and the time during which the cells were incubated with the phage/DOGS mixture. This method requires only several microliters of an Escherichia coli culture medium containing recombinant (f 1) phage particles and is applicable to various cell lines including mouse NIH/3 T3, chinese hamster (\mathrm{CHO}-\mathrm{K} 1), and human HT-1 080. 1994 Academic Press, Inc.