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Recombinant ELISA using baculovirus-expressed VP2 for detection of antibodies against canine parvovirus

โœ Scribed by Gabriella Elia; Costantina Desario; Giulia Pezzoni; Michele Camero; Emiliana Brocchi; Nicola Decaro; Vito Martella; Canio Buonavoglia


Book ID
113768942
Publisher
Elsevier Science
Year
2012
Tongue
English
Weight
256 KB
Volume
184
Category
Article
ISSN
0166-0934

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โœ Barbara Kropff; Maria-Paola Landini; Michael Mach ๐Ÿ“‚ Article ๐Ÿ“… 1993 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 827 KB

Two prokaryotically expressed fusion proteins encompassing amino acids 484-650 (AD-I) and 27-100 (AD-2) of glycoprotein gp58/116 of human cytomegalovirus (HCMV) were purified from E. coli lysates and used in ELISA to determine antibody levels in human sera. The specificity of the test was establishe