We investigated the effect of primer-template mismatch on the efficiency of polymerase chain reaction. For primers with T, C, or G as the 3 nucleotide, Thermus aquaticus (Taq) DNA polymerase was highly specific for template complementarity to this base, but was somewhat less constrained opposite the
β¦ LIBER β¦
Recognition of Remote Mismatches by DNA Polymerases
β Scribed by Michael Strerath; Jens Gaster; Andreas Marx
- Publisher
- John Wiley and Sons
- Year
- 2004
- Tongue
- English
- Weight
- 172 KB
- Volume
- 5
- Category
- Article
- ISSN
- 1439-4227
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