The effect of introducing an immobilized metal ion ligand in the lower phase of the PEG/Dextran system was studied on the erythrocytes and lymphocytes partition. The ligand in the lower phase was added as an insoluble form [Sepharose-IDA-M(II)] with or without a ligand in the upper phase. We first c
β¦ LIBER β¦
Recognition and separation of isoenzymes by metal chelates: Immobilized metal ion affinity partitioning of lactate dehydrogenase isoenzymes
β Scribed by Andreas Otto; Gerd Birkenmeier
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 887 KB
- Volume
- 644
- Category
- Article
- ISSN
- 1873-3778
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## Abstract Immobilized metalβion affinity partitioning (IMAP) is shown to be useful as a preliminary screening test and for the separation of different cell populations based upon recognition of the differences in the proteins on cell surfaces. The feasibility of using IMAP to segregate a spectrum