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Recognition and processing of the origin of transfer DNA by conjugative relaxase TrwC

✍ Scribed by Guasch, Alicia ;Lucas, María ;Moncalián, Gabriel ;Cabezas, Matilde ;Pérez-Luque, Rosa ;Gomis-Rüth, F Xavier ;de la Cruz, Fernando ;Coll, Miquel

Book ID
109965167
Publisher
Nature Publishing Group
Year
2003
Tongue
English
Weight
850 KB
Volume
10
Category
Article
ISSN
1545-9993

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✍ Coupland, George M. ;Brown, Anthony M. C. ;Willetts, Neil S. 📂 Article 📅 1987 🏛 Springer 🌐 English ⚖ 900 KB

The origin of transfer (oriT) is the sequence within which conjugal transfer of plasmid DNA is initiated, and is absolutely required in cis for plasmid mobilization. We have cloned oriT from the 52 kb IncN plasmid R46 on a 600 bp fragment, and mapped the limits of the relevant sequence by deletion a