𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Reciprocal changes in gene expression profiles of cocultured breast epithelial cells and primary fibroblasts

✍ Scribed by Patricia Bortman Rozenchan; Dirce Maria Carraro; Helena Brentani; Louise Danielle de Carvalho Mota; Elen Pereira Bastos; Elisa Napolitano e Ferreira; Cesar H. Torres; Maria Lúcia Hirata Katayama; Rosimeire Aparecida Roela; Eduardo C. Lyra; Fernando Augusto Soares; Maria Aparecida Azevedo Koike Folgueira; João Carlos Guedes Sampaio Góes; Maria Mitzi Brentani

Publisher
John Wiley and Sons
Year
2009
Tongue
French
Weight
428 KB
Volume
125
Category
Article
ISSN
0020-7136

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The importance of epithelial‐stroma interaction in normal breast development and tumor progression has been recognized. To identify genes that were regulated by these reciprocal interactions, we cocultured a nonmalignant (MCF10A) and a breast cancer derived (MDA‐MB231) basal cell lines, with fibroblasts isolated from breast benign‐disease adjacent tissues (NAF) or with carcinoma‐associated fibroblasts (CAF), in a transwell system. Gene expression profiles of each coculture pair were compared with the correspondent monocultures, using a customized microarray. Contrariwise to large alterations in epithelial cells genomic profiles, fibroblasts were less affected. In MDA‐MB231 highly represented genes downregulated by CAF derived factors coded for proteins important for the specificity of vectorial transport between ER and golgi, possibly affecting cell polarity whereas the response of MCF10A comprised an induction of genes coding for stress responsive proteins, representing a prosurvival effect. While NAF downregulated genes encoding proteins associated to glycolipid and fatty acid biosynthesis in MDA‐MB231, potentially affecting membrane biogenesis, in MCF10A, genes critical for growth control and adhesion were altered. NAFs responded to coculture with MDA‐MB231 by a decrease in the expression of genes induced by TGFβ1 and associated to motility. However, there was little change in NAFs gene expression profile influenced by MCF10A. CAFs responded to the presence of both epithelial cells inducing genes implicated in cell proliferation. Our data indicate that interactions between breast fibroblasts and basal epithelial cells resulted in alterations in the genomic profiles of both cell types which may help to clarify some aspects of this heterotypic signaling. © 2009 UICC

📜 SIMILAR VOLUMES

Profiling of differentially expressed ge
Profiling of differentially expressed genes induced by high linear energy transfer radiation in breast epithelial cells
✍ Debasish Roy; Gloria Calaf; Tom K. Hei 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 215 KB

Methods to define patterns of gene expression have applications in a wide range of biological systems. Several molecular biological techniques are used to study expression patterns during the neoplastic progression of breast epithelial cells. In the present study, differential expression of human on

Expression profiling of Wnt family of ge
Expression profiling of Wnt family of genes in normal and inflammatory bowel disease primary human intestinal myofibroblasts and normal human colonic crypt epithelial cells
✍ K.R. Hughes; F. Sablitzky; Y.R. Mahida 📂 Article 📅 2011 🏛 John Wiley and Sons 🌐 English ⚖ 169 KB 👁 2 views

Background: Wnt signaling regulates intestinal epithelial stem cell function. Wnt ligands bind Frizzled (Fz) receptors and lowdensity lipoprotein-receptor-related protein (LRP) 5 and 6. Secreted Frizzled-related protein (SFRP) and Dickkopf families inhibit Wnt signaling. Our aim was to study express

Differential expression of human ferriti
Differential expression of human ferritin H chain gene in immortal human breast epithelial MCF-10F cells
✍ Nadia A. Higgy; Ana M. Salicioni; Irma H. Russo; Pei Li Zhang; Jose Russo 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 470 KB 👁 2 views

Subtractive hybridization was used to isolate genes expressed uniquely in the immortalized human breast epithelial cell (HBEC) line MCF-10F and not in the mortal HBEC line S-130, from which MCF-10F cells were derived. We identified a 233-bp cDNA that was expressed in MCF-10F cells and not in their m

Protein expression profiling of primary
Protein expression profiling of primary mammary epithelial cells derived from MMTV-neu mice revealed that HER2/NEU-driven changes in protein expression are functionally clustered
✍ Sungwoo Park; Kyung-min Lee; Ji-hyun Ju; Jaeyoon Kim; Dong-Young Noh; Taehoon Le 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 252 KB

## Abstract MMTV‐__neu__ transgenic mice overexpressing NEU in their mammary glands develop tumor after 6 months of age. To find a novel protein biomarker using this mouse model, we identified and characterized the proteins that were differently expressed between primary mammary epithelial cells fr

Differential display analysis of breast
Differential display analysis of breast carcinoma cells enriched by immunomagnetic target cell selection: Gene expression profiles in bone marrow target cells
✍ Anne Hansen Ree; Olav Engebraaten; Eivind Hovig; Øystein Fodstad 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 French ⚖ 121 KB

## Abstract The red bone marrow (BM) is an important indicator organ of hematogenous micrometastatic spread of carcinomas. Characterization of biological properties specific for BM micrometastatic cells, however, is technically challenging due to the limited number of target cells usually available