Receptors for insulin and epidermal growth factor: Interaction with organomercurial agarose

✍ Scribed by Linda S. Aglio; Joseph M. Maturo III; Morley D. Hollenberg

• Book ID: 102878558
• Publisher: John Wiley and Sons
• Year: 1985
• Tongue: English
• Weight: 911 KB
• Volume: 28
• Category: Article
• ISSN: 0730-2312
• DOI: 10.1002/jcb.240280207

✦ Synopsis

The receptor for both insulin and epidermal growth factor (EGF) from human placental membranes, after crosslink labeling with 1251-labeled insulin and EGF, can be adsorbed to an organomercurial-agarose derivative (Affi-Gel 501) and can be recovered from the gel by elution with dithiothreitol (DTT). Pretreatment of crosslink-labeled membranes with N-ethylmaleimide (NEM) blocks the ability of the receptor to react with the organomercurial column. NEM also abolishes the protein kinase activity of both receptors. Under appropriate conditions, insulin can promote the reaction of the insulin receptor with the organomercurial-agarose derivative. For both the insulin and EGF receptors, our results provide an avenue for the isolation of the sulfhydryl-containing receptor domains that may play a role in the control of receptor function.