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Receptor-stimulated guanine-nucleotide-triphosphate binding to guanine-nucleotide-binding regulatory proteins : Nucleotide exchange and β-subunit-mediated phosphotransfer reactions

✍ Scribed by Sylvia KALDENBERG-STASCH; Michael BADEN; Barbara FESSELER; Karl H. JAKOBS; Thomas WIELAND


Book ID
115130728
Publisher
John Wiley and Sons
Year
1994
Tongue
English
Weight
964 KB
Volume
221
Category
Article
ISSN
1432-1327

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A method to study \(\left[\alpha^{32}\right.\) P \(]\) GTP binding to the \(\alpha\) subunit of GTP-binding proteins in rat brain membranes is described. This method measures receptor-stimulated GTP binding to individual \(\alpha\) subunits. GTP binding is associated with two protein bands following