Recently primed CD8+ T cells entering the liver induce hepatocytes to interact with naïve CD8+ T cells in the mouse

✍ Scribed by Nektarios Dikopoulos; Ursula Wegenka; Andrea Kröger; Hansjörg Hauser; Reinhold Schirmbeck; Jörg Reimann

• Publisher: John Wiley and Sons
• Year: 2004
• Tongue: English
• Weight: 411 KB
• Volume: 39
• Category: Article
• ISSN: 0270-9139
• DOI: 10.1002/hep.20173

✦ Synopsis

Large number of T cells traffic through the liver. In order to examine the effects of such traffic on the phenotype of hepatocytes, we vaccinated mice using DNA vaccines encoding antigens with MHC class I-binding epitopes. Small numbers of activated CD8 ؉ T blasts (10 5 -10 6 / liver) changed the surface phenotype and cytokine expression profile of hepatocytes (HCs).

HCs upregulate surface expression of major histocompatibility complex (MHC) class I molecules and CD1d but not MHC class II molecules

Qa-1, CD80, CD86, CD54, or CD95; in addition, they expressed/secreted interleukin (IL)-10 and IL-4 but not IL-1, IL-6, IL-13, interferon (IFN)-␥, tumor necrosis factor (TNF), IL-4, or IL-27 (i.e., they acquire the HC* phenotype). HCs* (but not HCs) induced specific activation, proliferation, and IFN-␥, TNF, and IL-13 release of cocultured naı ¨ve CD8 ؉ T cells. In contrast to the specific activation of naı ¨ve CD8 ؉ T cells by dendritic cells (DCs), specific CD8 ؉ T cell activation by HC* was not down-modulated by IFN-␣␤. Only recently activated CD8 ؉ T blasts (but not recently activated CD4 ؉ T blasts or activated cells of the innate immune system, including natural killer T [NKT] cells) induced the HC* phenotype that is prominent from day 10 to day 20 postvaccination (i.e., time points at which peak numbers of recently primed CD8 ؉ T blasts are found in the liver). In conclusion, recently activated CD8 ؉ T blasts that enter the liver postimmunization in small numbers can transiently modulate the phenotype of HC, allowing them to activate naı ¨ve CD8 ؉ T cells with unrelated specificities. (HEPATOLOGY 2004;39: 1256 -1266.