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Recent progress in the assays of xylanolytic enzymes

✍ Scribed by Peter Biely; Vladimír Puchart

Publisher
John Wiley and Sons
Year
2006
Tongue
English
Weight
233 KB
Volume
86
Category
Article
ISSN
0022-5142

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✦ Synopsis

Abstract

Increasing industrial importance of xylanolytic enzymes together with recent evidence for cereal endoxylanase inhibitors having a negative impact on the application of enzymes in fodder and food industries, brings challenges to facilitate and simplify the procedures to follow these enzymes. This article gives a critical evaluation of current methods and outlines new methodological alternatives to follow the major depolymerising enzyme, endo‐β‐1,4‐xylanase using chromogenic substrates. Attention is paid also to assays of three accessory xylanolytic enzymes α‐glucuronidase, acetylxylan esterase and feruloyl esterase, for which new enzyme‐coupled assays were elaborated. Copyright © 2006 Society of Chemical Industry

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