Recent progress in high-performance anion-exchange chromatography/ionspray mass spectrometry for molecular mass determination and characterization of carbohydrates using static and scanning array detection
✍ Scribed by van der Hoeven, R. A. M.; Tjaden, U. R.; van der Greef, J.; van Casteren, W. H. M.; Schols, H. A.; Voragen, A. G. J.; Bruggink, C.
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 336 KB
- Volume
- 33
- Category
- Article
- ISSN
- 1076-5174
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✦ Synopsis
Position-and time-resolved ion counting (PATRIC) array detection with a magnetic sector mass spectrometer equipped with an ionspray interface in the on-line coupling of high-performance anion-exchange chromatography (HPAEC) with mass spectrometry (MS) is described. PATRIC array detection is used in both the static and scanning mode for molecular mass determination and structure characterization caused by nozzle/skimmerinduced fragmentation in the ionspray interface. The virtual mass range is extended to over 7000 Da by the presence of triply and quadruply sodiated molecules in an enzyme digest of an exocellular polysaccharide (EPS) of Lactococcus lactis and quadruply sodiated molecules of b-1,4-xylose oligomers. After optimization of the HPAEC/MS system, molecular mass information could be obtained down to ¿700 pg per injection for each a-1,4-glucose oligomer up to a degree of polymerization of 7 using PATRIC array detection in the static mode. Structure information was obtained for a-1,4 glucose oligomers, galacturonic acid oligomers and an EPS digest using PATRIC array detection in the scanning mode.