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Recent developments in heterogeneous enzyme immunoassay

✍ Scribed by Carl Borrebaeck; Bo Mattiasson

Book ID
112964584
Publisher
Springer-Verlag
Year
1979
Tongue
English
Weight
484 KB
Volume
4
Category
Article
ISSN
0273-2289

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Inorganic pyrophosphatase from Escherichia coli has been employed as a label in heterogeneous enzyme immunoassays. Enzyme-antibody conjugates were prepared with the use of glutaraldehyde and purified by gel permeation chromatography. Enzyme activity was measured by means of a sensitive one-step colo